How is recombinant DNA technology helping in detecting the presence of mutant gene in cancer patients?
Answers
A single stranded DNA or RNA, tagged with a radioactive molecule is allowed to hybridize with its complementary DNA in a clone of cells followed by detection using auto-radiography.
The clone having the mutated gene will not appear on photographic film, because probe will not be complementary with mutated gene thus, helpful in detecting the presence of mutated gene in cancer patients.
The recombitant DNA technology refers to a technique that is helpful in detecting the cancer cells in a patient's body.
A single strand of DNA or RNA is tagged with radioactive molecules which are referred to as the probe therefore allowing it to hybridise.
This results in the complementary DNA to clone a number of the cells.
These cells are then detected through the process of autoradiography. the clone that contains the mutated gene isnt observed on the photographic film.