How is the 3 prime and the 5 prime end of the polynucleotide determined?
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Polynucleotide chains
At the other end, called the 3' end, the 3' hydroxyl of the last nucleotide added to the chain is exposed. DNA sequences are usually written in the 5' to 3' direction, meaning that the nucleotide at the 5' endcomes first and the nucleotide at the 3'end comes last
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