Question

How is the amplification of gene of interest carried out using pcr?

Answer 1

How is the amplification of a gene sample of interest carried out using Polymerase Chain Reaction (PCR)? Denaturation: Heating the double stranded DNA upto 98°C cause the separation of two DNA strands. Annealing: The two sets of primer are added which bind to appropriate complementary segment of DNA strands


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