Question

how is the stock of water calculated​

Answer 1

Answer:

Explanation:

To make a typical 100 microMolar (100X) stock concentration of primers, dissolve the primers in a volume of sterile distilled water that is 10X the amount of nmoles in the tube, using microliters of water. This value is printed on the side of the tube. For example, if your tube contains 53.4 nanomoles of primer, then you would dissolve using 534 microliters of water. This will now be at a 100 uM concentration.

then Dilute this stock 1:10, to give a concentration of 10 uM. store in -20 degree..

Answer 2

Explanation:

To make a typical 100 microMolar (100X) stock concentration of primers, dissolve the primers in a volume of sterile distilled water that is 10X the amount of nmoles in the tube, using microliters of water. This value is printed on the side of the tube.