Question

How many cycle of PCR are needed to produce approx 1/4 billion copy of gene

Answer 1

Answer:

PCR technique amplify the gene of interest in three steps namely denaturation of target DNA (thermal cycle to separate the DNA strands), annealing of primers to the ssDNA and polymerization (extension of primer into complete DNA strand complementary to the template strand). After completion of one cycle of PCR, two copies of the target DNA are produced both of which serve as a template for next PCR cycle and produce 4 copies. Hence, there is an exponential amplification of DNA copies. The total number of DNA molecules, produced after "n" PCR cycle = 2

n

. Thus, the total number of DNA molecules, produced after "6" PCR cycles = 2

6

= 64.

Answer 2

Answer:

After 30 cycles, as many as a billion copies of the target sequence are produced from a single starting molecule.