Question

How many days cells can be kept after dcfda staining?

Answer 1

Answer:

DCFH-DA is the most widely used probe for detecting intracellular H2O2 and oxidative stress. This probe is cell-permeable and is hydrolyzed intracellularly to the DCFH carboxylate anion, which is retained in the cell. ... DCFH does not directly react with H2O2 to form the fluorescent product, DCF

Explanation:

Reactive oxygen species (ROS) are chemically reactive chemical species containing oxygen. ... However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress