How many fragments were produced by the restriction enzyme hindiii?
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A linear phage chromosome is labeled at both ends with 32P and digested with restriction enzymes. EcoRI produces fragments of sizes 2.9, 4.5, 6.2, 7.4, and 8.0 kb. An autoradiogram developed from a Southern blot of this digest shows radioactivity associated with the 6.2 and8.0 fragments.
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