Question

How many Fragments will you get when you cut the following polypeptide with trypsin and chymotrypsin K-A-R-P-V-G-R-P-F-K-A-A-R-R-A
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Answer 1

Answer:

Explanation:

Trypsin cuts at lysine and arginine, while chymotrypsin. cuts at tyrosine, phenylalanine, and tryptophan.

Answer 2

There will be 8 fragments.

Explanation:

• Trypsin cuts peptide chains mainly at the carboxyl side of the amino acids lysine(K) or arginine(R).
• Chymotrypsin is a protease enzyme that cleaves on the C-terminal phenylalanine (F), tryptophan (W), and tyrosine (Y) on peptide chains.