How many rpm is generally used for shaking in rotary shaker incubator for oxygen transfer rate?
Answers
Maximum oxygen transfer rates (38 mmol liter−1 h−1, corresponding to a mass transfer coefficient of 188 h−1) were measured during orbital shaking at 300 rpm at a shaking diameter of 5 cm and a culture volume of 0.5 ml.
Explanation:
➡️ Bacterial liquid cultures should be shaken always (but not too vigorously), in order to ensure aeration and oxygen and nutrient availability as well as to avoid bacterial settlement on the flask bottom which would result in cell death from the lack of nutrient availability. Also, shaking prevents bacterial clumps or biofilm formation, ensuring prolific bacterial reproduction. Depending on the type of bacteria and the size of the flask, adjust rpm so that it's not too vigorous that it will damage the cells and prevent proper replication.
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