Question

How much amount of glycerol stock should be innoculated?

Answer 1

Add 0.5 ml yeast culture to 0.5 ml 30% (v/v) glycerol in 1.5 ml cryovials or sterile eppendorf tubes. 4. Invert tubes to mix cells and glycerol, then store at -80 °C.

Answer 2

Answer:

Protocol. Follow the steps for Inoculating an Overnight Liquid Culture. After you have bacterial growth, add 500 μL of the overnight culture to 500 μL of 50% glycerol in a 2 mL screw top tube or cryovial and gently mix. Note: Make the 50% glycerol solution by diluting 100% glycerol in dH20.