Question

How much dna concentration required forsequencing?

Answer 1

Answer:

here Ur Answer Buddy

Genomic DNA should be run on a gel to confirm integrity. Please provide gel image and 260/280 and 260/230 ratios at the time of submission. Methyl-Seq DNA Library Preparation. Whole Genome Bisulfite Sequencing (WGBS) and Reduced Representation Bisulfite Sequencing (RRBS) are possible.

Answer 2

Answer:

PCR for several times getting the same light band results. I have also tried using more of DNA template but still the concentration is too little. Is there any mean to get more PCR product? How do I concentrate the DNA? I used recombineering protocol to insert my interested gene into chromosomes of E.coli.