How one can apply the centrifugation techniques in isolating various cell organelles from kidney or from heart muscles. Explain the process in detail?
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The Basics of Centrifugation – Learn about the science behind centrifugation, common terms relating to the topic and must-know information about instruments themselves. This primer covers basic components of a centrifuge, operating principles, rotors, calculating centrifugal fields, separations, safety, and more.
Rotors and Sedimentation – Horizontal rotors and fixed rotors affect sedimentation differently. Horizontal rotors allow particles to deposit evenly on the bottom of the tube or container. Conversely, Fixed angle rotors allow particles to strike the opposite side of the tube then slide down the wall to the bottom. This results in faster sedimentation. The primer will discuss the difference between these rotors and their separation types.
Rotors and Sedimentation – Horizontal rotors and fixed rotors affect sedimentation differently. Horizontal rotors allow particles to deposit evenly on the bottom of the tube or container. Conversely, Fixed angle rotors allow particles to strike the opposite side of the tube then slide down the wall to the bottom. This results in faster sedimentation. The primer will discuss the difference between these rotors and their separation types.
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centrifugation is the process of separating collidal solutions .
it work on principal of centrifugal force.
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