How roots of woody plants are growing out in tissue culture?
Answers
Answered by
0
THE METHODOLOGY OF TISSUE CULTURE
The variety of techniques which can be used to get plant development in vitro (that is, by techniques such as tissue culture) is considerable and completely dependent upon the species in question. Single cells of leaf tissue can regenerate whole plants, as can shoot tips, leaf pieces, root pieces, lateral buds, or stem sections. Not all of these methods are applicable to woody plants, and indeed, not all have been applied to a commercial level to any plants.
Shoot tip culture is the method in widest use for the mass propagation of woody species. An actively growing shoot tip is surface sterilized and placed on a defined culture medium under sterile conditions. The culture medium contains inorganic and organic salts (macronutrients, micronutrients and vitamins) as well as an energy source (sucrose or table sugar), growth regulators, and agar )to gel the medium). If the growth regulators are appropriately balanced, the shoot tip elongates, lateral buds break and begin growth, and adventitious shoots are also produced on the stem piece. This rapid proliferation of shoots results in masses of shoots being produced from a single shoot tip. Up to a hundred shoots may be produced in as little as eight to twelve weeks from a single tip. The number of shoots produced and the rapidity of shoot proliferation varies between species, and in some cases, between cultivars of a single species. Shoots are removed from the cultures at regular intervals and a portion of the mass is replaced on fresh media to continue proliferation. The small shoots which are removed are then rooted in a separate medium, either a sterile gelled medium or a peat-perlite medium (in much the same fashion as conventional woody cuttings).
The variety of techniques which can be used to get plant development in vitro (that is, by techniques such as tissue culture) is considerable and completely dependent upon the species in question. Single cells of leaf tissue can regenerate whole plants, as can shoot tips, leaf pieces, root pieces, lateral buds, or stem sections. Not all of these methods are applicable to woody plants, and indeed, not all have been applied to a commercial level to any plants.
Shoot tip culture is the method in widest use for the mass propagation of woody species. An actively growing shoot tip is surface sterilized and placed on a defined culture medium under sterile conditions. The culture medium contains inorganic and organic salts (macronutrients, micronutrients and vitamins) as well as an energy source (sucrose or table sugar), growth regulators, and agar )to gel the medium). If the growth regulators are appropriately balanced, the shoot tip elongates, lateral buds break and begin growth, and adventitious shoots are also produced on the stem piece. This rapid proliferation of shoots results in masses of shoots being produced from a single shoot tip. Up to a hundred shoots may be produced in as little as eight to twelve weeks from a single tip. The number of shoots produced and the rapidity of shoot proliferation varies between species, and in some cases, between cultivars of a single species. Shoots are removed from the cultures at regular intervals and a portion of the mass is replaced on fresh media to continue proliferation. The small shoots which are removed are then rooted in a separate medium, either a sterile gelled medium or a peat-perlite medium (in much the same fashion as conventional woody cuttings).
Similar questions