Question

how steptokinase completes it's work of clot busting......the mechanism

Answer 1

To enact the law in its present form would only add a new, menacing weapon to the state's rapidly expanding surveillance mechanism.

Answer 2

Clotting time was about 15 seconds. Bubble rise to half the
height of the clot was taken at the end point. A new standard
curve was prepared for each experiment with dilutions of a
material having high activator activity. It was found advan-
tageous to use the same plasminogen for the standard curve as
was used for the experimental series and to add it to an amount of
streptokinase sufficient to yield maximum activator activity.
The reciprocal of the lysis times was plotted against relative
concentrations of the standard solution; the reciprocal lysis
time of the undiluted standard was assigned 100 activator units.
Unknown solutions were diluted to give lo- to 30-minute lysis
times and each unknown was assayed at two dilutions. Since
lysis times much shorter than 10 minutes can easily be obtained
in this assay by using larger amounts of activator, it is clear that
depletion of bovine plasminogen was not a limiting factor in these
experiments. Streptokinase alone, in the concentration range
used for activator formation, gave lysis times in excess of 8 hours.
Activator activity is expressed as units per ,ug of protein.
Protein Determination-The method of Lowry, Robert