Question

How the ligation of selected DNA is done with vector . biology class 12th ​

Answer 1

Answer:

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The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. For example, you can ligate a foreign DNA at the Bam H I site of tetracycline resistance gene in the vector pBR322. ... But, non- recombinants will grow on the medium containing both the antibiotics.

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