Question

How the plasmid DNA is obtained without genomic DNA contamination by alkaline lysis method?

Answer 1

Answer:

The most common method used for separating plasmid DNA from chromosomal DNA is the alkaline lysis method developed by Birnboim and Doly. They exploited the supercoiled nature and relatively small size of plasmid DNA to separate it from chromosomal DNA. First, cells are broken open under alkaline conditions.

Explanation:

Answer 2

Answer:

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Explanation:

Dear

You can remove genomic DNA contamination by using DNaseI, Reduce the time of incubation to avoid the destruction of plasmid DNA. Also you can test the protocol of the following paper: Andersson K, Hjorth R. Plasmid. 1985 Jan;13(1):78-80.

Isolation of bacterial plasmids by density gradient centrifugation in cesium trifluoroacetate (CsTFA) without the use of ethidium bromide.

Regards

Marius