Question

how the protein molecules separated by gel filtration techniques​

Answer 1

Answer:

Gel filtration is atechnique in which theseparation of components is based on the difference inmolecular weight or size. It is the simplest and mildest of all thechromatography techniques and separates molecules on the basis of differences in size.

Answer 2

Gel filtration is also known as size-exclusion chromatography or molecular-sieve chromatography. In this process, separation is based on the differing ability (due to differing molecular size) of molecules in the sample to enter the pores of the gel-filtration medium. The stationary phase in this technique consists of beads of a hydrated, sponge-like material that has pores of molecular dimensions and with a narrow range of sizes. When an aqueous solution, containing molecules of various sizes, is passed through a column containing such ‘molecular sieves,’ molecules that are larger than the pores of the filtration medium move quickly through the column. Smaller molecules enter the pores of the gel and move slowly through the column

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