How the two protein can be purified at the physiological ph , if they both has same mass but the pi of one is 6.5 that is different from other?
Answers
Protein Analysis / Protein Characterization. Proteins differ from each other in their size, molecular structure and physiochemical properties. Separation is typically done via electrophoresis where proteins are differentiated by size or mass, and isoelectric focusing, where protein are separated by charge.
Answer:
protein instability may have nothing to do with the basically of the protein: Histones are highly basic and highly soluble proteins. your protein may be misfolded - either due to the tag or simply because it is misfolded on the conditions you are using. your protein may adhere to certain types of plastic tubes (DNA LoBind are notorious), your protein may favor certain buffer conditions (MBP percipiates upon introduction of ion-pairing additives).