Question

How to Calculate Catalytic Efficiency Constant of an E.coli Enzyme in s-1M-1 units?

I have

Km= 15.1 mM

Vmax= 0.311 μmole/min

[E. coli] = 3.84 μg/mL

Enzyme amount used is 50 μL and the assay in total is 1000 μL

Molecular weight of enzyme = 540000

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Answer 1

Answer:

DNA molecules are tightly wound around proteins - called histone proteins - which provide structural support and play a role in controlling the activities of the genes. A strand 150 to 200 nucleotides long is wrapped twice around a core of eight histone proteins to form a structure called a nucleosome. The histone octamer at the centre of the nucleosome is formed from two units each of histones H2A, H2B, H3, and H4. The chains of histones are coiled in turn to form a solenoid, which is stabilised by the histone H1. Further coiling of the solenoids forms the structure of the chromosome proper.