Question

How to calculate concentration from peak area hplc?

Answer 1

fractionated an extract of plant and I took 15mg in 20ml solvent of a fraction and injected to HPLC, got a sharp peak with good resolution,

and I injected a standard compound of concentration 5mg/10ml got the same sharp peak and the same RT

I need to calculated the concentration of the compound that matched the same peak of the Std.

I used the equation conc. of sample = [Area sample/Area Std ] * conc. of Std and according to that equation the calculation showed to be

[16957864/171987]*0.5 = 49.3 mg/ml .. I am not sure if this is correct or not ?

please any confirmation for this calculations ? any comments and suggestions ?

I am confused about this results

I got the equation from this link

http://www.arlok.com/articles/High-Performance%20Liquid%20Chromatography.pdf

Thank you for your help