How to calculate concentration of nanoparticles through dls?
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i guess you want to calculate the conc of nanoparticles (nM/pM) in the colloidal solution. If yes and NP are spherical in shape, then you can use the following steps:
a) You know the weight of gold taken for NP synthesis (Au only, not gold salt) = W
b) Density of gold = 19.3 gcm-3
c) Now you calculate the volume of gold taken = W/9.320
d) Calculate the average size of nanoparticles using TEM (make sure nanoparticles are monodispersed) (2R)
e) Calculate the volume of a single nanoparticle (assuming sphere) = 4x(pi x R^3)/3 = V
f) You can calculate the number of particles in colloidal solution (N) = (W/9.32) / V
g) Calculate the conc of NP = N / Final volume of colloidal solution
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