How to calculate dilution factor used in dissolution calculation?
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Step Dilutions
Example: Make only 300 μL of a 1:1000 dilution, assuming the smallest volume you can pipette is 2 μL.Choose step DFs: Need a total dilution factor of 1000. ...Formula: Final Volume / Solute Volume = DF.Plug values in: (300 μL) / Solute Volume = 10.Rearrange: Solute Volume = 300 μL / 10 = 30 μL.
Example: Make only 300 μL of a 1:1000 dilution, assuming the smallest volume you can pipette is 2 μL.Choose step DFs: Need a total dilution factor of 1000. ...Formula: Final Volume / Solute Volume = DF.Plug values in: (300 μL) / Solute Volume = 10.Rearrange: Solute Volume = 300 μL / 10 = 30 μL.
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