How to calculate dna concentration using spectrophotometry
Answers
Answer:
To evaluate DNA purity, measure absorbance from 230nm to 320nm to detect other possible contaminants. The most common purity calculation is the ratio of the absorbance at 260nm divided by the reading at 280nm. Good-quality DNA will have an A260/A280 ratio of 1.7–2.0.
Explanation:
To determine the concentration of DNA in the original sample, perform the following calculation:
dsDNA concentration = 50 μg/mL × OD260 × dilution factor.
dsDNA concentration = 50 μg/mL × 0.65 × 50.
dsDNA concentration = 1.63 mg/mL.
Explanation:
DNA concentration is estimated by measuring the absorbance at 260nm, adjusting the A260 measurement for turbidity (measured by absorbance at 320nm), multiplying by the dilution factor, and using the relationship that an A260 of 1.0 = 50µg/ml pure dsDNA.