How to calculate encapsulation effiencey of nanoparticles?
Answers
Answer: Let's say you synthesize nanoparticles encapsulated with a protein. To calculate the encapsulation efficiency, you have to dilute a specific amount of NPs in a buffer. For example: PLGA nanoparticles in NaOH 0.1M.
First you have to calculate which is your theoretical protein concentration.
theoretical protein concentration= (initial protein amount, mg)*(concentration for dilution, mg/ml)/ (initial NPs amount, mg)
Initial protein and NPs amount are known, as you know what you weighted for NPs preparation. The encapsulation efficiency is calculated by several methods but the most reliable is BCA, if it's about a protein so, this method gives you a concentration of protein in NPs.
EE%= (Measurement obtained by BCA method, mg/ml)/ theoretical protein concentration, mg/ml)*100