Question

How to calculate km and vmax from raw data?the formula to use.

Answer 1

Could anyone please help me in calculating the Km and Vmax values of an enzyme (I am working on dihydrofolate reductase DHFR) when I have substrate/product inhibition?

I see the inhibition on (specific activity nmol/min/mg to substrate concentration µM) curve, with substrate concentrations near the theoretical Vmax. (I mean the calculated Vmax by ignoring the inhibition).