Question

How to calculate molar excess of crosslinker added to a protein complex?

Answer 1

Answer:

Ensure that Protein-SH is prepared and ready to combine with Protein-NH2 in step 5. 1. Prepare Protein-NH2 in Conjugation Buffer. 2. Add the appropriate amount of crosslinker to the protein solution. The concentration of the Protein-NH2 determines the crosslinker molar excess to use.

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Explanation:

Answer 2

Answer:

Ensure that Protein-SH is prepared and ready to combine with Protein-NH2 in step 5. 1. Prepare Protein-NH2 in Conjugation Buffer. 2. Add the appropriate amount of crosslinker to the protein solution. The concentration of the Protein-NH2 determines the crosslinker molar excess to use.

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Explanation: