How to calculate molecular weight of dna fragments?
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Heyy user
I m paras
here is ur answer
Step 1. DNA restriction
If you have a large DNA molecule, you will probably cut it into smaller fragments using a so-called DNA restriction enzyme.
Step 2. Gel electrophoresis
Then you will place properly prepared samples of the nucleic acid solution in the wells of an agarose gel electrophoresis system and apply a voltage for a specified amount of time.
In one of the wells, you also place a " DNA ladder": a sample that contains DNA fragments with known numbers of base pairs
I m paras
here is ur answer
Step 1. DNA restriction
If you have a large DNA molecule, you will probably cut it into smaller fragments using a so-called DNA restriction enzyme.
Step 2. Gel electrophoresis
Then you will place properly prepared samples of the nucleic acid solution in the wells of an agarose gel electrophoresis system and apply a voltage for a specified amount of time.
In one of the wells, you also place a " DNA ladder": a sample that contains DNA fragments with known numbers of base pairs
Answered by
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If the DNA is single-stranded, then multiply the number of bases by the average mass of a single base (330 g/mol) to get the approximate mass of the whole molecule.
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