how to calculate protease activity from absorbance
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working on a recombinant Protease enzyme from a Bacterial species. If anyone could please help in finding the best protocol for protease enzyme assay, I am trying to measure the enzyme activity using spectrophotometer and I am taking reading in the range of 0.223 to 0.8 by using casein as a substrate and incubation time of 10 min, how I can calculate the enzyme activity from these absorbance value.
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