How to calculate protein concentration for western blot?
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Cell lysates, membrane and nuclear lysates: load 20 to 30 µg of total protein per well. This may require some optimization depending on the expression level of the protein in the sample you are testing. Purified protein(recombinant or endogenous): load 10 to 100 ng of protein
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Cell lysates, membrane and nuclear lysates: load 20 to 30 µg of total protein per well. This may require some optimization depending on the expression level of the protein in the sample you are testing. Purified protein(recombinant or endogenous): load 10 to 100 ng of protein
hope this answer helps you
please mark it as brainliest
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