Question

How to calculate rate of absorption of a compound?

Answer 1

Answer:

First you have to get lambda max for that compound in suitable solvent ( the solvent is taken as reference).

You have to draw calibration curve for your working range at lambda max (get the calibration curve as linear as possible, regression constant (R2) value as near equal to 1) with your standard compound with different concentration.

Prepare your compound ready for that solvent (if the concentration of your compound is out of range then dilute the sample), never forget to add the dilution factor,while calculating the concentration.

to get maximum absorption is to reach the absorbance value is 2.

equation A = 2-log%T.

if your compound get higher absorbance than dilute it as such than you can get the maximum absorbance 2.

Regards