How to calculate substrate concentration from standard curve of product?
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You can determine this by multiplying the volume of substrate added to each well by the concentration of the substrate (1 mg/ml or 1 ug/ul) to give you a mass. Paranitrophenylphosphate (the substrate) has a formula weight of 275 g/mole.
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i think it will be, You can determine this by multiplying the volume of substrate added to each well by the concentration of the substrate (1 mg/ml or 1 ug/ul) to give you a mass. Paranitrophenylphosphate (the substrate) has a formula weight of 275 g/mole
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