Question

How to calculate the b parameter for ir spectroscopy?

Answer 1

The fundamental idea is simple. IR absorptions happen when the incoming light frequency matches the frequency of amolecular vibrational mode (i.e. the tone of the "ringing" of a molecule). There are other additional requirements and details you can read about. Pragmatically,1. you identify the IR-absorption features of your molecule.2. you find a feature in the wavelength range of your spectrometer that is relatively free from interference in your sample and take a spectrum.3. More molecules means less light gets through the sample, thus the size of the dip in the IR light intensity at the frequencies of your molecule's absorption feature is proportional to the concentration. This relationship is NOT linear, because the material always absorbs photons proportionally as the light traverses the sample.4. You quantify the change in peak size asa function of known concentrations. Thispart is all technology. It really does workwell to print the spectra out, cut them out, and weigh them. 5. Convert "Transmission" to"Absorption" to get a parameter which is linearly proportional to the concentration. Just go to Wikipedia for these formulas. If you are using nice software, click the button and do this stepbefore the previous one.6. Make a line through the various concentration vs. Absorbance experimentsyou ran and that line tells you the concentration of any Absorbance spectrum you get of that molecule in that instrument in similar samples of that exact thickness.
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