How to calculate the mean time take taken for all the starch to be digested completely if we are given time it took to be digested completely and temperature
Answers
Answer:
Practical 1.4 - Effect of temperature on the action of an enzyme
Procedure
Set up water baths at various temperatures (e.g. 0°C, 20°C, 40°C, 60°C and 80°C).
Add starch solution to 5 test tubes.
Add amylase solution to another 5 test tubes.
Place one starch and one amylase test tube into each water bath for 5 minutes - to allow the enzyme and substrate to reach the desired temperature.
Place 1 drop of iodine into each dimple on a spotting tile.
Add the amylase to the starch in the 0°C water bath.
Start the timer.
Every minute remove a sample of the starch-amylase solution and add it to a drop of iodine on the spotting tile.
Repeat step 8 until the iodine no longer changes colour - meaning that there is no starch present, in other words the amylase has broken all starch down.
Repeat steps 6-9 for each of the temperatures.
Record results.
Draw a graph to show the time taken for starch to be digested at different temperatures.