Question

How to calculate the molar extinction coefficient of a protein?

Answer 1

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●Multiply l by c and then divide A by the product to solve for molar absorptivity. For example: Using a cuvette with a length of 1 cm, you measured the absorbance of a solution with a concentration of 0.05 mol/L. The absorbance at a wavelength of 280 nm was 1.5●○

Answer 2

The calculated concentration, assuming the stated percentabsorptivity value, is as follows: (A / εpercent) × 10 = cmg/ml (1.346 / 6.6) × 10 = 2.039mg/mL Assuming a MW = 66,400, the molar extinction coefficientat 280nm for BSA is approximately 43,824M-1 cm-1.





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