How to calculate the rf value in chemistry practical, chromatography
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Materials Required
Real Lab Procedure
Take a Whatman filter paper strip and using a pencil draw a horizontal line 4cm from one end of the paper. Then draw another line lengthwise (verticallly) from the centre of the paper. Name the point at which the two lines intersect as P.
Using a fine capillary tube, put a drop of the mixture of red and blue inks at the point P. Let it dry in air.
Put another drop on the same spot and dry again, so that the spot is rich in the mixture.
Pour equal amounts of isopropyl alcohol and distilled water into a chromatographic chamber and mix it well using a glass rod. This is used as the solvent.
Suspend the filter paper vertically in the chromatographic chamber containing the solvent in such a way that the pencil line remains about 2cm above the solvent level.
Close the jar with its lid and keep it undisturbed.
Notice the rising solvent along with the red and blue inks. After the solvent has risen about 15 cm you will notice two different spots of blue and red colors on the filter paper.
plz mark as brainliest dear..
Real Lab Procedure
Take a Whatman filter paper strip and using a pencil draw a horizontal line 4cm from one end of the paper. Then draw another line lengthwise (verticallly) from the centre of the paper. Name the point at which the two lines intersect as P.
Using a fine capillary tube, put a drop of the mixture of red and blue inks at the point P. Let it dry in air.
Put another drop on the same spot and dry again, so that the spot is rich in the mixture.
Pour equal amounts of isopropyl alcohol and distilled water into a chromatographic chamber and mix it well using a glass rod. This is used as the solvent.
Suspend the filter paper vertically in the chromatographic chamber containing the solvent in such a way that the pencil line remains about 2cm above the solvent level.
Close the jar with its lid and keep it undisturbed.
Notice the rising solvent along with the red and blue inks. After the solvent has risen about 15 cm you will notice two different spots of blue and red colors on the filter paper.
plz mark as brainliest dear..
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