Question

how to Calculate values of Km and Vmax for TIM ?

Answer 1

The Michaelis constant, denoted by KM, is a measure of enzyme/substrate affinity. ... KM has the same units as substrate concentration and is equal to the substrate concentration when the velocity of the reaction is at half its maximum value.

Next, you will obtain the rate of enzyme activity as 1/Vo = Km/Vmax (1/[S]) + 1/Vmax, where Vo is the initial rate, Km is the dissociation constant between the substrate and the enzyme, Vmax is the maximum rate, and S is the concentration of the substrate.