How to calculate vmax and km in enzyme inhibition data?
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the Km of my enzyme is about 1µM. (in case I ignored the inhibition).
The specific activity with 5µM substrate is 2µM/min/mg. (in my case this is Vmax when I ignore the inhibition).
The specific activity with 10µ substrate is 1,7µM/min/mg. (it is lower here, that means here we have inhibition).
The specific activity with 20µM substrate is 1,5µM/min/mg. (again inhibition).
I don't know what is the right method to calculate the Vmax and Km for that enzyme and substrate with that inhibition phenomenon, waiting kindly for your guiding.
The specific activity with 5µM substrate is 2µM/min/mg. (in my case this is Vmax when I ignore the inhibition).
The specific activity with 10µ substrate is 1,7µM/min/mg. (it is lower here, that means here we have inhibition).
The specific activity with 20µM substrate is 1,5µM/min/mg. (again inhibition).
I don't know what is the right method to calculate the Vmax and Km for that enzyme and substrate with that inhibition phenomenon, waiting kindly for your guiding.
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