How to culture Amoeba
Answers
Answer:
Amoebas can be found freely living and thriving in shallow pond waters with organic material.
To view amoebas under the microscope, students will need the following:
A sample of water collected from a pond with organic material
Pondweed from a pond
Petri dish
A compound light microscope
Water
A dropper
For this technique, the student may either observe a sample of pond water directly to identify the organism or conduct a simple culture to grow and increase the number of amoebas.
Amoeba culture is a simple exercise that involves the following few steps:
Place a few pondweeds in a petri-dish and add some water to cover the weed
Leave the culture is a dark room for a few days and wait until a brown scum forms on the surface
Answer:
Upon arrival of Amoeba stock cultures, loosen the caps and immediately aerate the cultures by forcing air into the liquid using a clean pipet. Cultures should be kept at 18–22 °C (64–72 °F) and placed out of direct sunlight as they avoid light (negative phototaxic). Avoid moving the culture