Question

How to determine the protein content in inclusion body?

Answer 1

Answer:

In one aspect, the invention provides a method for calculating recombinant protein concentration in bacterial inclusion body (IB) harvests comprising

Answer 2

Explanation:

.cruzi mitochondrion protein that has a 20 residues hydrophobic region. I'm using a CodonPlus strain and expressing it into a pET28a vector in LB media, 20ºC, 0.2 uM IPTG  overnight. However, im getting only 1.3-1.7 mg/ml of protein and more than 70% of my protein is in my pellet.