How to determine the rate of reaction of polyphenol oxidase?
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The initial rate is the rate of the reaction at the start of the reaction, before it has begun to slow down. If you make a graph of absorbance on the y-axis versus reaction time on the x-axis, the initial rate is the slope of the line that is tangent to the curve at time zero. It doesn't matter what the absorbance value is, as long as it is measurable. If your reaction is too slow to measure the absorbance change with time accurately, then you should add more enzyme to speed it up. If the reaction goes so fast that you don't have enough time to measure the initial rate, use less enzyme. The accurate measurement of initial rates is crucial for Michaelis-Menten kinetic analysis.
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