how to differentiate between casein and albumin by performing heat coagulation test
Answers
Heat coagulation test of protein is a biochemical test performed to determine the presence of proteins like albumin and globulin in protein. Coagulation of proteins as a response to heat is a common phenomenon. The heat coagulation of proteins occurs in one of the two stages; denaturation and agglutination or the separation of the denatured protein in a particular form. The heating of coagulable proteins at their isoelectric pH, a series of changes occur to the proteins; dissociation of subunits or the quaternary structure, uncoiling of the polypeptide chains ultimately leading to the matting together of uncoiled polypeptide chains. Processes like coagulation and flocculation are superficial visible manifestations of changes that occur in proteins during the denaturation process.
Heat coagulation test of protein is a biochemical test performed to determine the presence of proteins like albumin and globulin in protein. Coagulation of proteins as a response to heat is a common phenomenon. The heat coagulation of proteins occurs in one of the two stages; denaturation and agglutination or the separation of the denatured protein in a particular form. The heating of coagulable proteins at their isoelectric pH, a series of changes occur to the proteins; dissociation of subunits or the quaternary structure, uncoiling of the polypeptide chains ultimately leading to the matting together of uncoiled polypeptide chains. Processes like coagulation and flocculation are superficial visible manifestations of changes that occur in proteins during the denaturation process.Coagulation of proteins is an irreversible process that is maximum at the isoelectric pH of the proteins. Heat coagulation of proteins is an important clinical test for the detection of proteinuria. It is simple and less time-consuming. Both qualitative and quantitative estimation of proteins can be done with the heat coagulation method. The quantitative analysis of coagulation can be performed by measuring the coagulum formed on the test tube.
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