Question

How to find dissolved and bound phosphorus in water sample?

Answer 1

Answer:

The dissolved phosphorus test measures that fraction of the total phosphorus which is in solution in the water (as opposed to being attached to suspended particles). It is determined by first filtering the sample, then analyzing the filtered sample for totalphosphorus.

Answer 2

Answer:

Monitoring phosphorus

1. Monitoring phosphorus is challenging because it involves measuring very low concentrations down to 0.01 milligram per liter (mg/L) or even lower. Even such very low concentrations of phosphorus can have a dramatic impact on streams. Less sensitive methods should be used only to identify serious problem areas.
2. While there are many tests for phosphorus, only four are likely to be performed by volunteer monitors.
3. The total orthophosphate test is largely a measure of orthophosphate. Because the sample is not filtered, the procedure measures both dissolved and suspended orthophosphate. The EPA-approved method for measuring total orthophosphate is known as the ascorbic acid method. Briefly, a reagent (either liquid or powder) containing ascorbic acid and ammonium molybdate reacts with orthophosphate in the sample to form a blue compound. The intensity of the blue color is directly proportional to the amount of orthophosphate in the water.
4. The total phosphorus test measures all the forms of phosphorus in the sample (orthophosphate, condensed phosphate, and organic phosphate). This is accomplished by first "digesting" (heating and acidifying) the sample to convert all the other forms to orthophosphate. Then the orthophosphate is measured by the ascorbic acid method. Because the sample is not filtered, the procedure measures both dissolved and suspended orthophosphate.
5. The dissolved phosphorus test measures that fraction of the total phosphorus which is in solution in the water (as opposed to being attached to suspended particles). It is determined by first filtering the sample, then analyzing the filtered sample for total phosphorus.
6. Insoluble phosphorus is calculated by subtracting the dissolved phosphorus result from the total phosphorus result.
7. All these tests have one thing in common they all depend on measuring orthophosphate. The total orthophosphate test measures the orthophosphate that is already present in the sample. The others measure that which is already present and that which is formed when the other forms of phosphorus are converted to orthophosphate by digestion.

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