Question

How to get single colony of bacteria on nutrient agar plates by spread plate method?

Answer 1

• Sterilize the wire loop.

• Cool the loop by touching it on the edge of the sterile agar plate.

• Dip the loop into the broth culture containing the mixture of bacteria.

• Lift the lid of the plate just enough to insert the loop. ...

• The loop has picked up thousands of bacteria which are spread out over the surface of the agar.