How to identify bacteria growth in uv spectrometry?
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Cell suspensions are turbid. Cells absorb and scatter the light. The higher the cell concentration, the higher the turbidity. Spectrophotometerscan measure intensity of light very accurately. The cell culture is placed in a transparent cuvette and the absorption is measured relative to medium alone. Optical density (OD) is directly proportional to the biomass in the cell suspension in a given range that is specific to the cell type. Using spectrophotometry for measuring the turbidity of cultures is known as turbidometry.
This has made spectrophotometry the methods of choice for measurements of bacterial growth and related applications. Spectrophotometry's drawback is its inability to provide an absolute count or distinguish between living and dead
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