Question

How to isolate nematodes from infected roots and leaves?

Answer 1

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Answer 2

Method to isolate nematodes from infected roots and leaves:

Step 1: Collect the root and leaves infected with nematodes.

Step 2: Wash the leaves and roots with distilled water till the debris and other impurities are removed.  

Step 3: Place the cleaned sample in the Petridish and add little amount of water to it.

Step 4: Tear the part of the root and leaves with dissecting needle where nematodes are seen. This will lead to the release of the nematode in the water.

Step 5: The released nematode can be isolated and used for further study.