Biology, asked by sundasgulzar2018, 3 months ago

how to make cdna from rna explain in detail

Answers

Answered by Anonymous
1

Answer:

  1. Prepare sample. RNA serves as the template in cDNA synthesis. ...
  2. Remove genomic DNA. Trace amounts of genomic DNA (gDNA) may be co-purified with RNA. ...
  3. Select reverse transcriptase. ...
  4. Prepare reaction mix. ...
  5. Perform cDNA synthesis. ...
  6. Prepare sample. ...
  7. Remove genomic DNA. ...
  8. Select reverse transcriptase.
Answered by Anonymous
1

Explanation:

  1. Prepare sample. RNA serves as the template in cDNA synthesis. ...
  2. Remove genomic DNA. Trace amounts of genomic DNA (gDNA) may be co-purified with RNA. ...
  3. Select reverse transcriptase. ...
  4. Prepare reaction mix. ...
  5. Perform cDNA synthesis. ...
  6. Prepare sample. ...
  7. Remove genomic DNA. ...
  8. Select reverse transcriptase.
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