how to make cdna from rna explain in detail
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Answered by
1
Answer:
- Prepare sample. RNA serves as the template in cDNA synthesis. ...
- Remove genomic DNA. Trace amounts of genomic DNA (gDNA) may be co-purified with RNA. ...
- Select reverse transcriptase. ...
- Prepare reaction mix. ...
- Perform cDNA synthesis. ...
- Prepare sample. ...
- Remove genomic DNA. ...
- Select reverse transcriptase.
Answered by
1
Explanation:
- Prepare sample. RNA serves as the template in cDNA synthesis. ...
- Remove genomic DNA. Trace amounts of genomic DNA (gDNA) may be co-purified with RNA. ...
- Select reverse transcriptase. ...
- Prepare reaction mix. ...
- Perform cDNA synthesis. ...
- Prepare sample. ...
- Remove genomic DNA. ...
- Select reverse transcriptase.
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