Question

how to prepare a complex to record the spectrum

Answer 1

Answer:

Fluorescence excitation spectrum

The fluorescence excitation spectrum characterizes the electron distribution of the molecule in the ground state. Excitation is equivalent to absorption since upon absorption, the molecule reaches the excited state Sn. The fluorescence excitation spectrum is obtained by fixing the emission wavelength and by running the excitation monochromator. Figure 2.9 displays the fluorescence excitation spectrum of Lens culinaris agglutinin. Fluorescence occurs from the Trp residues of the protein.