How to prepare lysis buffer for protein extraction?
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Perform all steps in a fume hood.
Prepare a 100 mM solution in double distilled water.
Set pH to 9.0 with HCl.
Boil until colorless. ...
Cool to room temperature.
Set pH to 9.0 again.
Boil until colorless.
Repeat this cycle until the solution remains at pH 9.0 after boiling and cooling.
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buffer used in Chromatin Immunoprecipitation (ChIP) Assays following initial cell lysis. Contains 1X PBS, 1% NP40, 0.5% Sodium Deoxycholate, 0.1% SDS & 1 Protease Inhibitor tablet. Free Antibody Samples. Receive Cruz Credits.
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