Question

How to prepare palmitic acid bsa conjugate for cell culture'?

Answer 1

Answer:

Explanation:

Supplemental – method for preparation of palmitic acid

Stock solution of palmitic acid was prepared by conjugating with fatty acid-free BSA as reported previously (1). In brief, palmitic acid was dissolved in pre-heated 0.1N NaoH and diluted 1:10 in prewarmed 12% BSA solution to give a final concentration of 10 mM. Control media contained 0.1N NaOH and BSA without lipid.

Reference:  

Sinha, S., Perdomo, G., Brown, N.F., O'Doherty, R.M. (2004) Fatty acid-induced insulin resistance in L6 myotubes is prevented by inhibition of activation and nuclear localization of nuclear factor kappa B. J Biol Chem 279, 41294-301