How to prepare sample for hplc analysis agilent for pesticides in water?
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PuriFlash 4250-250Specifications and Options
Prep HPLC 10 to 75mm ID
Flash Columns 4g - 800g
Normal & Reverse Phase
Flash and Prep HPLC in one instrument
Combine the simplicity of Flash purification with the power to run Prep HPLC columns into one instrument. Switch from normal to reverse phase in less than 10 seconds.
Intelligent Modular Design
The modular design of puriFlash instruments provides a level of flexibility to meet changing purification requirements. Upgrade at any time to a higher pressure or higher flow pump, additional detector (ELSD, RI, MS) or additional collector.
Touch Screen Controller
InterSoft 5.0, Free Upgrades
Solvent Level Monitoring
Leak Detection
4 USB Ports
Ethernet Ready
Save valuable hood space and locate the instrument on a bench with the fraction collector fume enclosure option.
Fraction Collector
4 Racks, 176 x 18mm Tubes
Full Size Keyboard
Solvent Resistant

HPLC Pump
True Quaternary Gradient
250 ml/min, 3625 PSI/250 bar
UV Detector
Dual Wavelength DAD
200 – 600 nm + Scanning
Spectral View & Purity Confirmation
Sample Loading
HPLC - Inline Loop Injection
Flash - Dry Load & Liquid Load
High Volume, Through-Pump
Multiple Injection Sequence
Integrated Column Holders
Flash and Prep HPLC
Column Switching Valve
PF4250-250: Part No. PF4250-250
Purification Based Software
Purification is a dynamic environment yet most Prep HPLC instruments rely upon analytical based software which restricts the user from making changes after starting the run. The puriFlash software allows the user to alter run conditions at anytime during equilibration and after the run has begun: gradient slope, flow rate, tube collection volume etc.
Switch from Flash to Prep HPLC in 10 seconds
Simply purge with propanol for 10 seconds and the instrument is ready to equilibrate a column in normal phase Flash or reverse phase Prep HPLC. With four solvent inlets (ABCD), many puriFlash users dedicate AB to reverse phase and CD to normal phase. Purging with propanol is as simple as clicking the purge button on the touch screen controller.
Prep HPLC 10 to 75mm ID
Flash Columns 4g - 800g
Normal & Reverse Phase
Flash and Prep HPLC in one instrument
Combine the simplicity of Flash purification with the power to run Prep HPLC columns into one instrument. Switch from normal to reverse phase in less than 10 seconds.
Intelligent Modular Design
The modular design of puriFlash instruments provides a level of flexibility to meet changing purification requirements. Upgrade at any time to a higher pressure or higher flow pump, additional detector (ELSD, RI, MS) or additional collector.
Touch Screen Controller
InterSoft 5.0, Free Upgrades
Solvent Level Monitoring
Leak Detection
4 USB Ports
Ethernet Ready
Save valuable hood space and locate the instrument on a bench with the fraction collector fume enclosure option.
Fraction Collector
4 Racks, 176 x 18mm Tubes
Full Size Keyboard
Solvent Resistant

HPLC Pump
True Quaternary Gradient
250 ml/min, 3625 PSI/250 bar
UV Detector
Dual Wavelength DAD
200 – 600 nm + Scanning
Spectral View & Purity Confirmation
Sample Loading
HPLC - Inline Loop Injection
Flash - Dry Load & Liquid Load
High Volume, Through-Pump
Multiple Injection Sequence
Integrated Column Holders
Flash and Prep HPLC
Column Switching Valve
PF4250-250: Part No. PF4250-250
Purification Based Software
Purification is a dynamic environment yet most Prep HPLC instruments rely upon analytical based software which restricts the user from making changes after starting the run. The puriFlash software allows the user to alter run conditions at anytime during equilibration and after the run has begun: gradient slope, flow rate, tube collection volume etc.
Switch from Flash to Prep HPLC in 10 seconds
Simply purge with propanol for 10 seconds and the instrument is ready to equilibrate a column in normal phase Flash or reverse phase Prep HPLC. With four solvent inlets (ABCD), many puriFlash users dedicate AB to reverse phase and CD to normal phase. Purging with propanol is as simple as clicking the purge button on the touch screen controller.
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